This is the deidentified individual-level analysis dataset from a test-negative design case control study in Medellin Colombia, to evaluate the efficacy of wMel Wolbachia releases in reducing dengue incidence. It underlies the analysis and results reported in the paper by Velez et al "Reduced dengue incidence following city-wide wMel Wolbachia mosquito releases throughout three Colombian cities: interrupted time series analysis and a prospective case-control study" published in PLoS Neglected Tropical Diseases in 2023.