Genome-wide SNP markers for 66 golden perch individuals of known sex (file GOP_Report_DMacq18-3638_sexing.csv), metadata for genotyping file (DART_Metadata_GOP.txt) and a covariate file for analyses using dartr package with geographic and sex data for each individual (file GOP_covariates_confident_gp_with_gonad_weights_genetic_sexing.csv). This dataset was used to find sex-linked markers for the manuscript by Pavlova A, Harrisson KA, Turakulov RI, Lee YP, Ingram B, Gilligan D, Sunnucks P, Gan HM. (2021) Labile sex chromosomes in the Australian freshwater fish family Percichthyidae. Molecular Ecology Resources.