Eltham copper butterfly Paralucia pyrodiscus: scoping genetic rescue

This repository includes the data, text and supplementary material for the manuscript:

Roitman, M., Gardner, M.G., New, T.R., Nguyen, T.T.T., Roycroft, E.J., Sunnucks, P., Yen, A.L., Harrisson, K.A., 2017. Assessing the scope for genetic rescue of an endangered butterfly: the case of the Eltham copper. Insect Conservation and Diversity 10, 399–414.

Final version of the manuscript:

Roitman_etal_ICD_final.docx

Supplementary Material for the manuscript:

icad12235-sup-0001-appendix.A.doc -- DNA extractions of larvae samples

icad12235-sup-0002-appendix.B.pdf -- Primer sequences

icad12235-sup-0003-appendix.C.doc -- Nuclear primer design

icad12235-sup-0004-appendix.D.pdf -- PCR conditions

icad12235-sup-0005-appendix.E.doc -- Summary of MIGRATE-N analysis

icad12235-sup-0006-appendix.F.doc -- Genetic diversity statistics

icad12235-sup-0007-appendix.G.doc -- Summary of *BEAST analysis in tree

form

icad12235-sup-0008-appendix.H.doc -- Summary of STRUCTURE analysis

based on all P. pyrodiscus samples

icad12235-sup-0009-appendix.I.pdf -- Sample information

icad12235-sup-0009-appendix.I Sample database in excel form.xls

Genotypes for nuclear sequencing data and results of GenAlEx analyses:

Heterozygosity nuclear_KH.xlsx

Mitochondrial sequencing data, in nexus format (‘traits’ are locations):

Concatenated CO1 ND5 mitochondria.nex

Nucleotide alignment CO1ND5_concatenated_traits.nex

Nucleotide alignment CO1_Ktrimmed.nex

Nucleotide alignment CO1_Ktrimmed_traits.nex

Nucleotide alignment ND5_Ktrimmed.nex

Nucleotide alignment ND5_Ktrimmed_traits.nex

Phased nuclear sequences for five anonymous single copy nuclear loci, in nexus format:

63EA_phased.nex

A7D8_phased.nex

S5ZW_phased_updated.nex

O3W6_phased_updated.nex

Z5V8_phased.nex