Data to support TCRa and TCRb repertoire analysis of human thymocyte subsets (samples fk_167, fk_168 and fk_172)
datasetposted on 23.01.2019, 10:52 authored by Stephen DaleyStephen Daley
The file contains human T cell receptor (TCR) sequences obtained by multiplex PCR amplification of cDNA molecules followed by Illumina sequencing. Sequences were aligned to the human genome using MIGEC software (see doi: 10.1038/nmeth.2960 for details). Except for the header row, each row contains information about a unique TCR nucleotide sequence. Column 1 stores the TCR chain (a, alpha; b, beta). Column 2 stores the T cell subset. Column 3 is an identifier for the thymus sample of origin. Columns 4 and 5 store the nucleotide sequence and amino acid sequence, respectively, of the complementarity-determining region 3 (CDR3). Columns 6 and 7 store the TCR variable (v) and joining (j) gene segment information.