<p>The
file contains mouse T cell receptor (TCR) sequences obtained by multiplex PCR
amplification of cDNA molecules followed by Illumina sequencing. Sequences were
aligned to the mouse genome using MIGEC software (see doi: 10.1038/nmeth.2960
for details). Sequences with a read count of <3 were excluded. Except for
the header row, each row contains information about a unique TCR nucleotide
sequence. Columns 1-11 contain output from MIGEC software. Columns 12-15
contain information about sample origin, detailed as follows: column 12
"sample_id" is an identifier for the sample of origin; column 13
"tcr_b" specifies the TCR beta chain status of donor mouse
("poly" = C57BL/6 inbred mouse strain); column 14 “mouse” is an
identifier for the individual mouse of origin and column 15 "subset"
specifies the T cell subset of origin ("t_1" = Type A IELp
thymocytes; "t_4" = CD4+ naive thymocytes; "t_8" = CD8+
naive thymocytes). For materials and methods refer to Wirasinha et al Immunol Cell Biol. 2018 Jul;96(6):553-561. doi:
10.1111/imcb.12047. PMID: 29726044.</p>
Funding
National Health and Medical Research Council grant 1107464