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Data from: The influence of immune activation on thermal limits along a latitudinal cline

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posted on 2020-01-28, 02:39 authored by Tobias HectorTobias Hector, Carla sgrò, Matthew HallMatthew Hall
When using this data please cite the original publication:


Files in this package:

Title: static_heat_data.csv
Description: Experiment 1: data from 39 degrees static heatshock assay
File contains:
"dose" = the immune challenge ("H10" = high dose, "M9" = medium dose, "L8" = low dose, "PBS" = wounded, "CTRL" = unwounded controls).
"assay_time" = time in hours between immune challenge and the assay ("3h", "6h", "12h" & "24h").
"block_num" = experimental block
"run_num" = experimental assay run
"death_time" = knockdown time in minutes


Title: ramp_data.csv
Description: Experiment 1: data from two ramping thermal assays both starting at 25 degrees
File contains:
"dose" = the immune challenge ("H10" = high dose, "M9" = medium dose, "L8" = low dose, "PBS" = wounded, "CTRL" = unwounded controls).
"ramp_rate" = ramping rate of thermal assays ("0.1" & "0.06" degrees per minute).
"block_num" = experimental block
"run_num" = experimental assay run
"ctmax" = CTmax (degrees) for each individual


Title: cold_data.csv
Description: Experiment 1: data from chill coma recovery assay. Recovery time at 25 degrees was measured after 5 hours exposure to zero degrees
File contains:
"dose" = the immune challenge ("H10" = high dose, "M9" = medium dose, "L8" = low dose, "PBS" = wounded, "CTRL" = unwounded controls).
"ramp_rate" = assay type "cold".
"block_num" = experimental block
"run_num" = experimental assay run
"recovery time" = recovery time in minutes after being returned to 25 degrees

Title: immune_cline_data.csv
Description: Experiment 2: data from static heat shock at 39 degrees on individuals from 12 populations of Drosophila originating from along a latitudinal cline that were exposed to wounding or bacterial immune challenge.
File contains:
"population" = population ID's for the 12 populations
"longitude" = longitudinal place of origin for each of the 12 populations of Drosophila (rounded to nearest integer)
"treatment" = immune challenge treatment ("CTRL" = unwounded controls, "PBS" = wounded, "INF" = bacteria immune challenge).
"gen_num" = Drosophila generation number used in experiment
"run_num" = experimental assay run
"block" = experimental block
"death_time" = knockdown time in minutes in 39 degree heatshock assay
"wounding_effect" = relative effect of wounding on knockdown time (only relevant for "PBS" treatment group)
"immune_effect" = relative effect of bacterial immune challenge on knockdown time (only relevant for "INF" treatment group).

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    Keywords

    Global change biologyDrosophila melanogasterlocal adaptationthermal stressinfectious diseaseCTmaximmune challengethermal limitshost-pathogen interactionsimmune activationthermal adaptationAnimal ImmunologyAnimal Physiological EcologyBiological AdaptationEcological PhysiologyEvolutionary BiologyEvolutionary Impacts of Climate ChangeGlobal Change BiologyHost-Parasite InteractionsImmunologyInfectious Diseases

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