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Data from: Pathogen exposure disrupts an organism’s ability to cope with thermal stress

dataset
posted on 09.07.2019 by Tobias Hector, Carla Sgrò, Matthew Hall
When using this data, please cite the original publication:

Hector, T.H., Sgrò C. and M. D. Hall (2019) Pathogen exposure disrupts an organism's ability to cope with thermal stress. Global Change Biology. doi: 10.1111/gcb.14713

Files in this package:

Title: hector_et_al_experiment1_heatshock_data.csv
Description: Data from experiment 1 heatshock assay assessing knockdown times at 37 degrees.
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate date,
knockdown_time_mins = individual knockdown times (mins)

Title: hector_et_al_experiment1_ramp_data.csv
Description: Data from experiment 1 ramp assays assessing ctmax across different temperature ramping rates.
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate date,
ramping_rate = assay temperature ramping rate (0.06, 0.04 and 0.02 degrees per minute),
ctmax = individual CTmax (degrees celsius).

Title: hector_et_al_experiment2_heatshock_full_data.csv
Description: Data from experiment 2 heatshock assay assessing knockdown times across stages of infection (days post infection).
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate,
dpi_fct = days post infection ("10", "20", "30", "40" & "50), knockdown_time_mins = individual knockdown times (mins)

Title: hector_et_al_experiment2_ramp_full_data.csv
Description: Data from experiment 2 ramp assay assessing ctmax across stages of infection (days post infection).
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate,
dpi_fct = days post infection ("10", "20", "30", "40" & "50),
ctmax = individual CTmax (degrees celsius).

Title: hector_et_al_experiment2_heatshock_data_infected.csv
Description: Data from experiment 2 heatshock assay used for analysing how spore loads, relative host body size and days post infection impact relative knockdown times (relative to uninfected controls).
File contains:
host_gen = host genotype ("H" & "M")
pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run = assay replicate
dpi_fct = days post infection ("20", "30", "40" & "50),
body_size = raw individual body size
knockdown_time_mins = raw individual knockdown times
ctrl_time = mean control knockdown times for each host genotype by dpi combination
ctrl_size = mean control body size
relative_time = knockdown times relative to corresponding control mean
relative_size = body size relative to corresponding control mean
mature_spores_mil = individual pathogen spore counts (millions)

Title: hector_et_al_experiment2_ramp_data_infected.csv
Description: Data from experiment 2 ramp assay used for analysing how spore loads, relative host body size and days post infection impact relative CTmax (relative to uninfected controls).
File contains:
host_gen = host genotype ("H" & "M")
pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run
dpi_fct = days post infection ("20", "30", "40" & "50),
body_size = raw individual body size
knockdown_time_mins = raw individual knockdown times
ctrl_time = mean control knockdown times for each host genotype by dpi combination
ctrl_size = mean control body size
relative_ctmax = ctmax relative to corresponding control mean
relative_size = body size relative to corresponding control mean
mature_spores_mil = individual pathogen spore counts (millions)

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