Data from: Pathogen exposure disrupts an organism’s ability to cope with thermal stress

2019-07-09T04:38:58Z (GMT) by Tobias Hector Carla Sgrò Matthew Hall
When using this data, please cite the original publication:

Hector, T.H., Sgrò C. and M. D. Hall (2019) Pathogen exposure disrupts an organism's ability to cope with thermal stress. Global Change Biology. doi: 10.1111/gcb.14713

Files in this package:

Title: hector_et_al_experiment1_heatshock_data.csv
Description: Data from experiment 1 heatshock assay assessing knockdown times at 37 degrees.
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate date,
knockdown_time_mins = individual knockdown times (mins)

Title: hector_et_al_experiment1_ramp_data.csv
Description: Data from experiment 1 ramp assays assessing ctmax across different temperature ramping rates.
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate date,
ramping_rate = assay temperature ramping rate (0.06, 0.04 and 0.02 degrees per minute),
ctmax = individual CTmax (degrees celsius).

Title: hector_et_al_experiment2_heatshock_full_data.csv
Description: Data from experiment 2 heatshock assay assessing knockdown times across stages of infection (days post infection).
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate,
dpi_fct = days post infection ("10", "20", "30", "40" & "50), knockdown_time_mins = individual knockdown times (mins)

Title: hector_et_al_experiment2_ramp_full_data.csv
Description: Data from experiment 2 ramp assay assessing ctmax across stages of infection (days post infection).
File contains:
host_gen = host genotype ("H" & "M"),
pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"),
assay_run = assay replicate,
dpi_fct = days post infection ("10", "20", "30", "40" & "50),
ctmax = individual CTmax (degrees celsius).

Title: hector_et_al_experiment2_heatshock_data_infected.csv
Description: Data from experiment 2 heatshock assay used for analysing how spore loads, relative host body size and days post infection impact relative knockdown times (relative to uninfected controls).
File contains:
host_gen = host genotype ("H" & "M")
pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run = assay replicate
dpi_fct = days post infection ("20", "30", "40" & "50),
body_size = raw individual body size
knockdown_time_mins = raw individual knockdown times
ctrl_time = mean control knockdown times for each host genotype by dpi combination
ctrl_size = mean control body size
relative_time = knockdown times relative to corresponding control mean
relative_size = body size relative to corresponding control mean
mature_spores_mil = individual pathogen spore counts (millions)

Title: hector_et_al_experiment2_ramp_data_infected.csv
Description: Data from experiment 2 ramp assay used for analysing how spore loads, relative host body size and days post infection impact relative CTmax (relative to uninfected controls).
File contains:
host_gen = host genotype ("H" & "M")
pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run
dpi_fct = days post infection ("20", "30", "40" & "50),
body_size = raw individual body size
knockdown_time_mins = raw individual knockdown times
ctrl_time = mean control knockdown times for each host genotype by dpi combination
ctrl_size = mean control body size
relative_ctmax = ctmax relative to corresponding control mean
relative_size = body size relative to corresponding control mean
mature_spores_mil = individual pathogen spore counts (millions)