10.26180/5d24476ddf364
SARAH ALYCE REVITT-MILLS
SARAH ALYCE
REVITT-MILLS
Functional and genetic characterisation of Tcp plasmid maintenance and conjugation systems in Clostridium perfringens
Monash University
2019
Plasmid
Clostridium perfringens
Conjugation
Plasmid Stability
pCW3
STED
Microbiology
2019-07-09 07:51:08
Thesis
https://bridges.monash.edu/articles/thesis/Functional_and_genetic_characterisation_of_Tcp_plasmid_maintenance_and_conjugation_systems_in_Clostridium_perfringens/8832383
Clostridium perfringens (italics) is an important pathogen in both humans and animals. The disease-causing capabilities of this bacterium lie in its arsenal of 20 or more toxins and enzymes, many of which are located on DNA elements called plasmids. This study investigates how these plasmids transfer between C. perfringens (italics) cells and how they are maintained within this bacterium. This work has characterised two systems that are essential for plasmid maintenance in C. perfringens (italics) and has identified two new components of the plasmid transfer system. This work has also used super-resolution microscopy to map the location of components of the plasmid transfer system on the bacterial cell.