Data from: Pathogen exposure disrupts an organism’s ability to cope with thermal stress HectorTobias SgròCarla HallMatthew 2019 <div><b>When using this data, please cite the original publication:</b></div><div><br></div>Hector, T.H., Sgrò C. and M. D. Hall (2019) Pathogen exposure disrupts an organism's ability to cope with thermal stress. Global Change Biology. doi: 10.1111/gcb.14713<div><br></div><div><div><b>Files in this package:</b></div><div><br></div><div>Title: hector_et_al_experiment1_heatshock_data.csv</div><div>Description: Data from experiment 1 heatshock assay assessing knockdown times at 37 degrees. </div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"),</div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate date, </div><div>knockdown_time_mins = individual knockdown times (mins)</div><div><br></div><div><div>Title: hector_et_al_experiment1_ramp_data.csv</div><div>Description: Data from experiment 1 ramp assays assessing ctmax across different temperature ramping rates. </div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"), </div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate date, </div><div>ramping_rate = assay temperature ramping rate (0.06, 0.04 and 0.02 degrees per minute), </div><div>ctmax = individual CT<sub>max</sub> (degrees celsius).</div></div><div><br></div><div><div>Title: hector_et_al_experiment2_heatshock_full_data.csv</div><div>Description: Data from experiment 2 heatshock assay assessing knockdown times across stages of infection (days post infection). </div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"), </div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate, </div><div>dpi_fct = days post infection ("10", "20", "30", "40" & "50), knockdown_time_mins = individual knockdown times (mins)</div></div></div><div><br></div><div><div>Title: hector_et_al_experiment2_ramp_full_data.csv</div><div>Description: Data from experiment 2 ramp assay assessing ctmax across stages of infection (days post infection).</div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"), </div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate, </div><div>dpi_fct = days post infection ("10", "20", "30", "40" & "50),</div><div>ctmax = individual CT<sub>max</sub> (degrees celsius).</div></div><div><br></div><div><div>Title: hector_et_al_experiment2_heatshock_data_infected.csv</div><div>Description: Data from experiment 2 heatshock assay used for analysing how spore loads, relative host body size and days post infection impact relative knockdown times (relative to uninfected controls). </div><div>File contains:</div><div>host_gen = host genotype ("H" & "M")</div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run = assay replicate</div><div>dpi_fct = days post infection ("20", "30", "40" & "50), </div><div>body_size = raw individual body size</div><div>knockdown_time_mins = raw individual knockdown times</div><div>ctrl_time = mean control knockdown times for each host genotype by dpi combination</div><div>ctrl_size = mean control body size</div><div>relative_time = knockdown times relative to corresponding control mean</div></div><div>relative_size = body size relative to corresponding control mean</div><div>mature_spores_mil = individual pathogen spore counts (millions)</div><div><br></div><div><div><div>Title: hector_et_al_experiment2_ramp_data_infected.csv</div><div>Description: Data from experiment 2 ramp assay used for analysing how spore loads, relative host body size and days post infection impact relative CTmax (relative to uninfected controls). </div><div>File contains:</div><div>host_gen = host genotype ("H" & "M")</div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run </div><div>dpi_fct = days post infection ("20", "30", "40" & "50), </div><div>body_size = raw individual body size</div><div>knockdown_time_mins = raw individual knockdown times</div><div>ctrl_time = mean control knockdown times for each host genotype by dpi combination</div><div>ctrl_size = mean control body size</div><div>relative_ctmax = ctmax relative to corresponding control mean</div></div><div>relative_size = body size relative to corresponding control mean</div><div>mature_spores_mil = individual pathogen spore counts (millions)</div></div>