10.26180/5ceb7e7939b25 Tobias Hector Tobias Hector Carla Sgrò Carla Sgrò Matthew Hall Matthew Hall Data from: Pathogen exposure disrupts an organism’s ability to cope with thermal stress Monash University 2019 Thermal limits thermal tolerance infectious disease aquatic ectotherm Global Change Biology Evolutionary Impacts of Climate Change Host-Parasite Interactions Infectious Diseases 2019-07-09 04:38:58 Dataset https://bridges.monash.edu/articles/dataset/Data_from_Pathogen_exposure_disrupts_an_organism_s_ability_to_cope_with_thermal_stress/8187815 <div><b>When using this data, please cite the original publication:</b></div><div><br></div>Hector, T.H., Sgrò C. and M. D. Hall (2019) Pathogen exposure disrupts an organism's ability to cope with thermal stress. Global Change Biology. doi: 10.1111/gcb.14713<div><br></div><div><div><b>Files in this package:</b></div><div><br></div><div>Title: hector_et_al_experiment1_heatshock_data.csv</div><div>Description: Data from experiment 1 heatshock assay assessing knockdown times at 37 degrees. </div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"),</div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate date, </div><div>knockdown_time_mins = individual knockdown times (mins)</div><div><br></div><div><div>Title: hector_et_al_experiment1_ramp_data.csv</div><div>Description: Data from experiment 1 ramp assays assessing ctmax across different temperature ramping rates. </div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"), </div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate date, </div><div>ramping_rate = assay temperature ramping rate (0.06, 0.04 and 0.02 degrees per minute), </div><div>ctmax = individual CT<sub>max</sub> (degrees celsius).</div></div><div><br></div><div><div>Title: hector_et_al_experiment2_heatshock_full_data.csv</div><div>Description: Data from experiment 2 heatshock assay assessing knockdown times across stages of infection (days post infection). </div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"), </div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate, </div><div>dpi_fct = days post infection ("10", "20", "30", "40" & "50), knockdown_time_mins = individual knockdown times (mins)</div></div></div><div><br></div><div><div>Title: hector_et_al_experiment2_ramp_full_data.csv</div><div>Description: Data from experiment 2 ramp assay assessing ctmax across stages of infection (days post infection).</div><div>File contains: </div><div>host_gen = host genotype ("H" & "M"), </div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), </div><div>assay_run = assay replicate, </div><div>dpi_fct = days post infection ("10", "20", "30", "40" & "50),</div><div>ctmax = individual CT<sub>max</sub> (degrees celsius).</div></div><div><br></div><div><div>Title: hector_et_al_experiment2_heatshock_data_infected.csv</div><div>Description: Data from experiment 2 heatshock assay used for analysing how spore loads, relative host body size and days post infection impact relative knockdown times (relative to uninfected controls). </div><div>File contains:</div><div>host_gen = host genotype ("H" & "M")</div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run = assay replicate</div><div>dpi_fct = days post infection ("20", "30", "40" & "50), </div><div>body_size = raw individual body size</div><div>knockdown_time_mins = raw individual knockdown times</div><div>ctrl_time = mean control knockdown times for each host genotype by dpi combination</div><div>ctrl_size = mean control body size</div><div>relative_time = knockdown times relative to corresponding control mean</div></div><div>relative_size = body size relative to corresponding control mean</div><div>mature_spores_mil = individual pathogen spore counts (millions)</div><div><br></div><div><div><div>Title: hector_et_al_experiment2_ramp_data_infected.csv</div><div>Description: Data from experiment 2 ramp assay used for analysing how spore loads, relative host body size and days post infection impact relative CTmax (relative to uninfected controls). </div><div>File contains:</div><div>host_gen = host genotype ("H" & "M")</div><div>pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run </div><div>dpi_fct = days post infection ("20", "30", "40" & "50), </div><div>body_size = raw individual body size</div><div>knockdown_time_mins = raw individual knockdown times</div><div>ctrl_time = mean control knockdown times for each host genotype by dpi combination</div><div>ctrl_size = mean control body size</div><div>relative_ctmax = ctmax relative to corresponding control mean</div></div><div>relative_size = body size relative to corresponding control mean</div><div>mature_spores_mil = individual pathogen spore counts (millions)</div></div>