Protein-based functional nanoparticles: Comparision of strategies for design and engineering PRADEEP G C 10.26180/5bc9535f71260 https://bridges.monash.edu/articles/thesis/Protein-based_functional_nanoparticles_Comparision_of_strategies_for_design_and_engineering/7228085 This Ph.D. thesis focuses on engineering protein-based functional nanoparticles that have attractive structural and functional properties. Different strategies in designing and engineering such nanoparticles have been studied and compared. The strategies include: i) covalent immobilisation of protein on silica nanoparticles, ii) non-covalent affinity immobilisation of proteins on silica and iron oxide nanoparticles, iii) self-assembly peptides for formation of protein-only nanoparticles, and iv) combination of self-assembly and affinity adsorption to form clusters of hybrid protein-iron oxide nanoparticles. This research has built a new platform for designing protein-based functional nanomaterials that may have diverse application in biomedical, health, and environmental sectors. 2018-10-19 03:45:33 Protein Purification Protein Immobilisation Fluorescence Nanoparticles Self-assembly Peptides Affinity Binding Peptides Iron-oxide Nanoclusters Bio-and Hybrid Nanoparticles Proteins and Peptides Biocatalysis and Enzyme Technology Nanobiotechnology